I confused myself during studying, and wanted to confirm something. Since transcription via RNA polymerases only takes place in the 5'to 3' direction, that would mean that that 5' to 3' strand is the only one that contains the information to be translated later into a protein or mRNA or w/e.
So then the 3' to 5' strand would NOT contain any genes, but just the base pair complement to them? Or am I thinking of the concept of a gene wrong?
Answer
There is nothing called the 3' to 5' strand. Both strands are have the same polarity but the DNA helix is anti-parallel. Both the strands contain approximately equal number of genes. Sometimes the transcription from both the strands can overlap, leading to production of antisense-transcripts.
So RNA polymerase will read the other strand from its 3' to 5'.
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