Wednesday 14 October 2015

terminology - Is a DNA molecule a single strand of polynucleotide or two of them linked together?


Our molecular biology teacher told us that a double helix of DNA was composed of two DNA molecules linked together by hydrogen bonds. The thing is, until now, I always thought a DNA molecule was composed of two strands, those being polynucleotides, both of them being linked together. I can't find a link which is saying the same as my teacher, even if it seems technically correct to call a double helix a dimer of two DNA molecules.


I was curious to know what was the exact terminology.




Answer



As you pointed out, though this may be basic biology, seeking clarification when receiving conflicting information is a good thing. Don't feel embarrassed for asking. :)





.. our molecular biology teacher told us that a double helix of DNA was composed of two DNA molecules linked together by hydrogen bonds.



Respectfully, your teacher is incorrect. A single, double-stranded DNA molecule is comprised of two helical shaped polynucleotides, and are connected together via hydrogen bonding.



Highlight of each polynucleotide


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Highlight of hydrogen bonding


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And just for further validation, according to Molecular Biology of the Cell, 4th ed., by Alberts B, Johnson A, Lewis J, et al.:



A DNA molecule consists of two long polynucleotide chains composed of four types of nucleotide subunits. Each of these chains is known as a DNA chain, or a DNA strand. Hydrogen bonds between the base portions of the nucleotides hold the two chains together.



So, it would seem that your teacher is referring to each polynucleotide, a.k.a. DNA strand, as a DNA molecule. Instead, she should use the verbiage: a single DNA molecule is composed of two DNA strands, which are helical-shaped polynucleotides.


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