As a follow-up of a previous question, I would like to know what enzymes or protein complexes have been used to manipulate DNA samples into stabilizing DNA loops.
I have read that cohesin is one of such enzymes, but works as a transcription factor by binding specific DNA motifs.
My interest is in knowing what other enzymes will create DNA loops with no DNA motif biases from a DNA extraction.
This is a follow-up question to this one, as ultimately I would like to know if there could be any ways of optimizing the process of DNA circularization of very long stretches of human (or mammalian) double-stranded DNA, from several kilobases to megabases, by first creating stable DNA loops that leave two DNA ends close by for a more effective ligation process.
Any ideas?
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