Sunday, 5 February 2017

plant anatomy - How can I stain only the Chitin but not Cellulose or Glucans?


Is there any stain, that binds specifically to chitin but not to cellulose? (for differential staining and double staining purpose)


It seems the lactophenol-cotton blue, aniline blue, trypan blue etc binding cellulosic plant cell wall and various other materials, so is there any such stain that more specifically bind to chitin and aminosugars like N-acetylglucosamine but does not easily bind to cellulose and other non-nitrogenous cell wall components?


P.S. Immunotechniques may be one solution but it is not available to me at this moment. Looking for chemical stains





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