Tuesday 8 November 2016

biochemistry - How is the rate of transcription influenced by temperature?


How is the rate of transcription influenced by temperature? More precisely, I am looking for an article who quantitatively measured the rate of transcription of an "average gene" and show how this rate is influenced by temperature.


On this question @Chris found this article where such measurement were performed but they were using some arbitrary unit (as it as been pointed out by @shigeta here) that cannot be related to any comprehensive unit such as "mole of amino acid per hours" or something like that.



Answer



If your looking at transcription then your talking about RNA POLYMERASE.


And there are many variants. Here's a good Nature paper that discusses temperature and RNA Pol



http://www.nature.com/ncomms/journal/v1/n6/full/ncomms1076.html


And another:


http://www.ncbi.nlm.nih.gov/m/pubmed/12729734/


I couldn't get full access to this JBC paper but I think the answer would be clearly in here, prob worth a look


M. Chamberlin and J. Ring. Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme. J Biol Chem. 248: 2235-44 (1973)


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