Wednesday, 30 March 2016

cellular respiration - Is glycolysis the beginning part of fermentation, or does fermentation follow glycolysis?


Is glycolysis the beginning part of fermentation, or does fermentation follow glycolysis?


I see conflicting information from different sources


https://honchemistry.wikispaces.com/Lactic+Acid+and+Alcohol+Fermentation+in+Humans




"..Alcohol fermentation follows glycolysis, just like lactic acid fermentation..." <-- So glycolysis is preceding fermentation, not part of fermentation



and



" The Actual Fermentation Part


Glycolysis and fermentation are two separate processes. Glycolysis was explained briefly to give the reader an idea of the events leading up to fermentation and the starting conditions in terms of molecules available for reaction.....Going into the fermentation, the molecules NADH and pyruvic acid are present. " <--- so glycolysis is preceding fermentation, not part of fermentation.



Whereas these two links put glycolysis as part of fermentation, not a preceding stage before fermentation.


https://www.khanacademy.org/science/biology/cellular-respiration-and-fermentation/variations-on-cellular-respiration/a/fermentation-and-anaerobic-respiration




"Fermentation and cellular respiration begin the same way, with glycolysis" <-- glycolysis is part of fermentation, not preceding it.



and


http://study.com/academy/lesson/anaerobic-respiration-lactic-acid-alcoholic-fermentation.html



"fermentation, which is a process that anaerobically generates ATP by performing glycolysis..." <-- fermentation includes glycolysis, so glycolysis is part of fermentation, not preceding it.



So, which is it?


Is it a)like khanacademy and study.com, or b)like honchemistry.wikispaces.com


I have heard the idea that glycolysis is independent, in that it can produce some (not much, but some), energy on its own, and it can happen without fermentation or cellular respiration following. Though when would glycolysis occur without fermentation or respiration following? And even if glycolysis is that independent, it may still be at the beginning of respiration and fermentation and not preceding it.



Wikipedia speaks in a contrary way..


https://en.wikipedia.org/wiki/Fermentation



"Before fermentation takes place, one glucose molecule is broken down into two pyruvate molecules. This is known as glycolysis." <-- so glycolysis is preceding fermentation, not part of it.



so the above quote from wikipedia suggests that glycolysis is not the beginning part of fermentation, but a step preceding it


whereas still on the fermentation wikipedia page, it says



"Fermentation is a metabolic process that converts sugar to acids, gases, or alcohol....The first step, glycolysis" <-- so glycolysis is part of fermentation.




Links on respiration are more unambiguous that glycolysis is part of respiration.. the links on fermentation vary a bit on whether glycolysis precedes fermentation or is part of it.



Answer



As @bpedit indicates in his comment, this is a semantic question — i.e. one regarding the meaning and usage of words. I will explain how I and others use these words and why. If you are convinced by my logic you will wish to use them in the same way, if not you are free to use them differently. However there is no ‘universal truth’ here, and if you are student I have no idea what your instructor thinks.


The problem is that ‘fermentation’ and ‘ferment’ (both as a noun and a verb) are old words, predating any understanding of the processes involved. Thus the Oxford English Dictionary quotes a relevant early example of the use of ‘ferment’ as a verb as follows:



1663 Cowley Verses, to Royal Society iv, All their juyce did .. Ferment into a .. refreshing Wine.



From this, it is clear that the idea of the fermentation process is the conversion of some initial compound (the sugar in the ‘juyce’) to some final compound (the alcohol in the wine). This usage is still current in referring to the process of fermentation, for example a Google search brings up a BBC Science educational page which states:



Beer and wine are alcoholic drinks made by fermentation reactions that use yeast to convert sugars into ethanol.




You may or may not be aware that term ‘ferment’ was historically used to mean what we now term yeast, and ‘Zwischenferment’, the obsolete German term for certain enzymes in yeast, is derived from this. So the original concept was merely of some biological conversion effected by yeast, although this has later been extended to bacteria and the idea changed to indicate metabolic processes capable of generating ATP anaerobically.


Part of the discrepancy in definition may be the emphasis on the end-product of the fermentation — ethanol fermentation, lactic acid fermentation etc. However, it seems clear to me that the fermentation is the whole chain of metabolic events from sugar (or whatever) to alcohol (or whatever); and if glycolysis is part of that process (which it is in this example) then glycolysis is part of the fermentation. (Of course, glycolysis can occur in other circumstances where the product, pyruvate, is oxidised in the tricarboxylic acid cycle, which is not a fermentation process.)


Footnote


The extent to which one can tie oneself in knots with trying to define fermentation is illustrated in the best-selling and well-respected text book, originally written by Lubert Streyer and subsequently maintained by Berg et al. I mention this text both because an older edition is available on-line, and because a book of such value to the publishers is carefully vetted by referees. In the chapter on Glycolysis there is an attempt to define Fermentation:



Fermentation: An ATP-generating process in which organic compounds act as both donors and acceptors of electrons. Fermentation can take place in the absence of O2. Discovered by Louis Pasteur, who described fermentation as “la vie sans l’air” (“life without air”).



So the biochemical lawyers have produced a definition that very few readers will be able to take in at first sight. What is this business about electron donors and acceptors? Well what it means in relation to the fermentation process in which lactic acid is produced (note my legalistic choice of words) is that one organic compound is reduced (glyceraldehyde 3-phosphate) — by NAD+ — and one organic compound is oxidized (pyruvate) — by NADH. And as the production of ATP is included in the definition this means that Berg et al. include glycolysis in this definition of fermentation.


…except that on the same page there is the following statement:




pyruvate is converted, or fermented, into lactic acid in lactic acid fermentation or into ethanol in alcoholic fermentation



So here it seems that the word is being used for the conversion of pyruvate to lactate or ethanol, i.e. it excludes glycolysis.


Pasteur managed to talk about fermentation without being aware of glycolysis or ATP, and it is clear to me that you can write whatever carefully phrased definitions you like, but people are going to continue to use venerable terms like fermentation in whatever way seems natual to them.


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