Thursday, 14 December 2017

biochemistry - Why do Type II Restriction Endonucleases cleave at palindromic sequences?


Type II Restriction enzymes usually cut only at palindromic sequences. Is there any specific reason for that? Is there any advantage for bacteria if they cleave phage DNA at this type of sequence?



Answer



First, not all restriction enzymes cut at palindromic sequences. A lot of them do though, simply because it is more effective. Recognising a palindromic sequence enables them to cut both strands of DNA at the "same" site, because the strand will have the same sequence only in different directions at that site.


See Wikipedia for example.



No comments:

Post a Comment

evolution - Are there any multicellular forms of life which exist without consuming other forms of life in some manner?

The title is the question. If additional specificity is needed I will add clarification here. Are there any multicellular forms of life whic...