biochemistry - Effect of testosterone hormone on cell signalling and behaviour?

A steroid, testosterone was injected in female body which led to development of secondary sexual characters but these characters were not developed over night (Response was very slow). What could be possible explanation of this phenomenon

1. a Steroid hormones produce a slow response because they do not have secondary messenger


2. Slow response is due to lack of cell membrane receptors and amplification enzymes


3. Movement of this hormone is very slow in blood


4. None of the above

I think option 3 is correct because steroid hormones are hydrophobic in nature and cant be transported in the blood without the help of any carrier protein so this may be the reason but I am not sure about this..

Answer

1. Steroid hormones produce a slow response because they do not have secondary messenger
   It's true that steroids don't have secondary messengers because they don't need them - they can go directly into a cells nucleus and affect transcription there. This wouldn't slow down the response by much, however.


2. Slow response is due to lack of cell membrane receptors and amplification enzymes
   This is just the inverse of the first option, which again isn't really a cause of slowing down the response. Steroids are amplified in that they act directly on DNA at the very first step of the synthesis pathways so don't need much amplification.


3. Movement of this hormone is very slow in blood
   Again I don't think there is much to this option, the difference in speed of distribution of steroid and peptide hormones is likely to be minimal

This leaves us with:
4. None of the above

I would personally have gone with this answer. Steroids have a long time of action because they require the new transcription of genes that they activate. This process takes time and consistent doses are needed to maintain the effect.