Sunday, 14 July 2019

genetics - Why don't restriction endonucleases digest transformed plasmids?


In the textbook that I'm using, it explains that bacteria does not digest its own chromosomal DNA because the sites that would be cut by its own endonuclease are methylated. Is there a similar mechanism involved for protecting its own plasmids? If so, how are plasmids cut while serving as vectors?




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